7,119 research outputs found
Microsomal nucleoprotein particles from pea seedlings
Ultracentrifugal analysis of an extract of pea epicotyls, previously freed of debris and larger particles by centrifugation at 40,000 g for 10 minutes, has revealed the presence of a major component which possesses a sedimentation coefficient of 74 S. This component constitutes about 25 per cent of the TCA-precipitable material in the clarified epicotyl extract and is estimated to make up 1 to 2 per cent of the dry weight of the original tissue. In size, chemical composition, and morphology, the 74 S component resembles the nucleoproteins of the microsomes from animal tissues.
The 74 S component of pea epicotyl extracts has been purified by repeated cycles of differential centrifugation to yield a preparation which is 80 per cent homogeneous in the analytical ultracentrifuge. It has been found to contain 30 to 37 per cent RNA as judged by a variety of analytical techniques. Approximately 55 per cent of the weight of the material is protein and a further 4.5 per cent phospholipide.
Electron micrographs of air-dried specimens of the purified preparation show the 74 S constituent to be flattened spheres with an average height of 180 A and an average diameter of approximately 280 A. The molecular weight of the 74 S particles is computed from sedimentation, viscosity, and partial specific volume data to be 4.5 million ± 10 per cent in agreement with the value estimated from electron micrographs.
The 74 S or microsomal component of pea epicotyls is rapidly aggregated in the presence of low concentrations of Mg ions or by somewhat higher concentrations of Ca or K salts. ATP on the contrary causes resolution of electrolyte-induced microsomal aggregates with simultaneous degradation of the particles to an ultracentrifugally inhomogeneous mixture of lower molecular weight materials
A study of wing body blending for an advanced supersonic transport
Increases in supersonic cruise lift drag ratio were sought at Mach numbers 2.2 and 2.7 using wing body planform and thickness blending. Constrained twist and camber optimization was performed in the presence of nacelles. Wing and fuselage thickness distributions were optimized for either minimum volume wave drag or minimum total pressure wave drag. The zero leading edge suction lift drag ratios were determined for three wing planforms. The magnitude of the effect of leading edge suction on attainable lift drag ratio was defined on one planform and estimation of available leading edge suction was made
The Supreme Court and Integration
The subject of this thesis was a result of the present school integration crisis that was occurring at the time it was written in 1964. The paper examines rights granted in the Thirteenth, Fourteenth, and Fifteenth Amendments to the Constitution, Supreme Court decisions regarding school integration, and the particular situation of school integration in Gadsden, Alabama. Analysis of integration cases prior to 1900, the Supreme Court\u27s 1954 decision, the situation in Little Rock, Arkansas, integration of Alabama Schools, and integration issues in other parts of the US is included
Crystal Structure and Magnetism of the Linear-Chain Copper Oxides Sr5Pb3-xBixCuO12
The title quasi-1D copper oxides (0=< x =<0.4) were investigated by neutron
diffraction and magnetic susceptibility studies. Polyhedral CuO4 units in the
compounds were found to comprise linear-chains at inter-chain distance of
approximately 10 A. The parent chain compound (x = 0), however, shows less
anisotropic magnetic behavior above 2 K, although it is of substantially
antiferromagnetic (mu_{eff}= 1.85 mu_{B} and Theta_{W} = -46.4 K) spin-chain
system. A magnetic cusp gradually appears at about 100 K in T vs chi with the
Bi substitution. The cusp (x = 0.4) is fairly characterized by and therefore
suggests the spin gap nature at Delta/k_{B} ~ 80 K. The chain compounds hold
electrically insulating in the composition range.Comment: To be published in PR
The Origin of Primordial Dwarf Stars and Baryonic Dark Matter
I present a scenario for the production of low mass, degenerate dwarfs of
mass via the mechanism of Lenzuni, Chernoff & Salpeter (1992).
Such objects meet the mass limit requirements for halo dark matter from
microlensing surveys while circumventing the chemical evolution constraints on
normal white dwarf stars. I describe methods to observationally constrain this
scenario and suggest that such objects may originate in small clusters formed
from the thermal instability of shocked, heated gas in dark matter haloes, such
as suggested by Fall & Rees (1985) for globular clusters.Comment: TeX, 4 pages plus 2 postscript figures. To appear in Astrophysical
Journal Letter
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